Cloned (Comment) | Organism |
---|---|
gene pmdE | Comamonas testosteroni |
gene pmdE, expression of wild-type and mutants in Escherichia coli | Comamonas testosteroni |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of several pmdE gene truncated mutants, lacking residues 6 to 226, 32 to 296, or 105 to 215, overview. A pmdE knock out mutant loses the ability to grow on vanillate, 3-hydroxybenzoate, and 4-hydroxybenzoate as carbon sources. This ability to grow on vanillate, 3-hydroxybenzoate, and 4-hydroxybenzoate is restored by genetic complementation | Comamonas testosteroni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | D0IZN8 | gene pmdE | - |
Comamonas testosteroni | Q93PS9 | gene pmdE | - |
Comamonas testosteroni CNB-1 | Q93PS9 | gene pmdE | - |
Comamonas testosteroni CNB-2 | D0IZN8 | gene pmdE | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:3-hydroxybenzoate-grown cell | - |
Comamonas testosteroni | - |
culture condition:4-hydroxybenzoate-grown cell | - |
Comamonas testosteroni | - |
culture condition:gentisate-grown cell | - |
Comamonas testosteroni | - |
culture condition:phenol-grown cell | - |
Comamonas testosteroni | - |
culture condition:vanillate-grown cell | - |
Comamonas testosteroni | - |
additional information | strain CNB-1 grows on phenol, gentisate, vanillate, 3-hydroxybenzoate, and 4-hydroxybenzoate as carbon and energy sources, but does not grow on resorcinol, 2,4-dihydroxybenzoate, 4-cresol, and phenylacetate | Comamonas testosteroni | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-4-carboxy-hexa-2,4-dienedioate | 4-oxalomesaconate hydratase uses only enol-OMA as substrate | Comamonas testosteroni | 4-carboxy-4-hydroxy-2-oxoadipate + H2O | - |
? | |
2-hydroxy-4-carboxy-hexa-2,4-dienedioate | 4-oxalomesaconate hydratase uses only enol-OMA as substrate | Comamonas testosteroni CNB-2 | 4-carboxy-4-hydroxy-2-oxoadipate + H2O | - |
? | |
2-hydroxy-4-carboxy-hexa-2,4-dienedioate | 4-oxalomesaconate hydratase uses only enol-OMA as substrate | Comamonas testosteroni CNB-1 | 4-carboxy-4-hydroxy-2-oxoadipate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OMA | - |
Comamonas testosteroni |
PmdE | - |
Comamonas testosteroni |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Comamonas testosteroni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at, about | Comamonas testosteroni |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Comamonas testosteroni | sequence calculation | - |
5.81 |
Comamonas testosteroni | isoelectric focusing | - |
6.23 |
General Information | Comment | Organism |
---|---|---|
evolution | comparison of gene clusters of the protocatechuate 4,5-cleavage pathway in bacterial strains, overview | Comamonas testosteroni |
malfunction | a pmdE knock out mutant loses the ability to grow on vanillate, 3-hydroxybenzoate, and 4-hydroxybenzoate as carbon sources. This ability to grow on vanillate, 3-hydroxybenzoate, and 4-hydroxybenzoate is restored by genetic complementation | Comamonas testosteroni |
metabolism | the enzyme is active in the protocatechuate 4,5-cleavage pathway. Gene pmdU encodes an 4-oxalomesaconate tautomerase and catalyzes conversion of 4-oxalomesaconate-keto into 4-oxalomesaconate-enol, which is the only substrate of 4-oxalomesaconate hydratase. Pathway overview | Comamonas testosteroni |
physiological function | the enzyme in strain CNB-1 is essential for growth on phenol, gentisate, vanillate, 3-hydroxybenzoate, and 4-hydroxybenzoate as carbon and energy sources | Comamonas testosteroni |